New views of tapetum ultrastructure and pollen exine development in Arabidopsis thaliana

Gene Regulatory Network for Tapetum Development in Arabidopsis thaliana

In flowering plants, male gametophyte development occurs in the anther. Tapetum, the innermost of the four anther somatic layers, surrounds the developing reproductive cells to provide materials for pollen development. A genetic pathway of DYT1-TDF1-AMS-MS188 in regulating tapetum development has been proven. Here we used laser microdissection and pressure catapulting to capture and analyze the...

Arabinogalactan Proteins in Arabidopsis thaliana Pollen Development

Identification of kaonashi Mutants Showing Abnormal Pollen Exine Structure in Arabidopsis thaliana

Exine, the outermost architecture of pollen walls, protects male gametes from the environment by virtue of its chemical and physical stability. Although much effort has been devoted to revealing the mechanism of exine construction, still little is known about it. To identify the genes involved in exine formation, we screened for Arabidopsis mutants with pollen grains exhibiting abnormal exine s...

Female Gametophyte Development and Pollen Tube Guidance in Arabidopsis thaliana

1 Department of Botany, Graduate School of Science, Kyoto University, Kitashirakawa-oiwake, Sakyo, Kyoto, 606-8502 Japan 2 Institute of Plant Biology, University of Zurich, Zollikerstrasse 107, Zurich, CH-8008, Switzerland 3 Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604 4 Department of Biological Mechanisms and Functions, Graduate School o...

Transcription factor AtMYB103 is required for anther development by regulating tapetum development, callose dissolution and exine formation in Arabidopsis.

Downregulation of the transcription factor AtMYB103 using transgenic technology results in early tapetal degeneration and pollen aberration during anther development in Arabidopsis thaliana. This paper describes the functional analysis of the AtMYB103 gene in three knock-out mutants. Two male sterile mutants, ms188-1 and ms188-2, were generated by ethyl-methane sulfonate (EMS) mutagenesis. A ma...